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deaD [2017-05-24 10:04:18]
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deaD [2017-05-24 10:04:18]

DEAD-box RNA helicase, important for adaptation to low temperatures
locus
BSU39110
pI
7.71
mw
53.85 kDa
protein length
479 aa Sequence Blast
gene length
1437 bp Sequence Blast
function
RNA helicase
product
DEAD-box RNA helicase
essential
no
synonyms
yxiN

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.3|DEAD-box RNA helicases]
  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.12|Translation/ other]
  • Gene

    Coordinates
    4,015,987 → 4,017,426

    The protein

    Catalyzed reaction/ biological activity

  • RNA helicase
  • Protein family

  • [SW|DEAD-box RNA helicases]
  • Paralogous protein(s)

  • [protein|E169F9711635457EA65FA71CCE9D7FF29DD8DF02|CshA], [protein|C83BD80DE0FD187D07B914F64696438ED5130107|CshB], [protein|0362D937D985846AEE4D0DEA57B62F915E8D35E5|YfmL]
  • [SW|Domains]

  • Helicase domain 1: AS 1-203
  • Linker region: AS 204-212
  • Helicase domain 2: AS 207-368
  • RNA-binding domain: AS 404-479
  • Structure

  • [PDB|2G0C] (RNA-binding domain, AA 404-479), [PDB|2HJV] (second domain, AA 207-368); [PDB|3MOJ] (RNA binding domain complexed with a fragment of 23S ribosomal RNA) [Pubmed|20673833]
  • Expression and Regulation

    Operons

    genes
    [gene|D2790591E8C3B86BF91F6E1B6DADBA306D5EF758|yxiM]-[gene|EACD0C84AA3CE41EF05EAA3D25AA1153293EB562|deaD]
    description
    [Pubmed|10746760]

    regulation

  • expression declines in the stationary phase [Pubmed|23175651]
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    Biological materials

    Mutant

  • MGNA-B720 (deaD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1719 NBRP B. subtilis, Japan]
  • GP1052 (tet), available in the [SW|Stülke] lab
  • Expression vector

  • for expression/ purification from ''B. subtilis'' with C-terminal Strep-tag, for [SW|SPINE], expression from the native chromomsomal site: GP1065 (spc), available in [SW|Jörg Stülke]'s lab
  • two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab
  • FLAG-tag construct

  • GP1068 (spc, based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab
  • Labs working on this gene/protein

  • [SW|Dagmar Klostermeier], Biozentrum Basel, Switzerland [http://www.biozentrum.unibas.ch/klostermeier/index.html homepage]
  • References

  • 17142894,16118224,17229151,16611943,19839642,19474341,18184816,17951299,12460566,10481020,20572937,20673833,20691700,23175651,23625962,25907111,25123660